PacBio HiFi long reads provide high accuracy to quickly and affordably generate contiguous, complete, and correct de novo genome assemblies.| PacBio
Is it possible to generate truly high-quality, long-read data from minuscule amounts of DNA? Absolutely, thanks to our new kit for ultra-low DNA input projects.| PacBio
New SPRQ chemistry for the Revio system is revolutionizing HiFi sequencing with increased efficiency, affordability, and reduced DNA input. Learn about its impact on research in rare diseases, cancer, and multiomics.| PacBio
Museum collections harbor millions of samples, largely unutilized for long-read sequencing. Here, we use ethanol-preserved samples containing kilobase-sized DNA to show that amplification-free protocols can yield contiguous genome assemblies. Additionally, using a modified amplification-based protocol, employing an alternative polymerase to overcome PCR bias, we assemble the 3.1 Gb maned sloth genome, surpassing the previous 500 Mb protocol size limit. Our protocol also improves assemblies of...| BioMed Central