Transfection is a core technique in molecular biology used to introduce foreign nucleic acids—such as DNA, RNA, or small RNAs like siRNA, shRNA, and miRNA—into eukaryotic cells. This enables researchers to manipulate gene expression and study cellular processes, disease mechanisms and therapeutic strategies (1). Advances in transfection technology now support a range of nucleic acid […] The post Why mRNA Transfection Is Transforming Transient Expression Workflows appeared first on Prome...| Promega Connections
August 2, 2023 Today we are happy to announce the launch of a new lungs-targeted in vivo transfection reagent. The reagent is crafted to transport small molecules, DNA, RNA, and proteins to the lungs tissue, aiming to treat lung diseases, including cystic fibrosis and lung cancer. The transfection reagent is a non-viral complex comprising nanoparticle-sized […] The post Lungs-Targeted Transfection Reagent: Safe and Effective Delivery of Biomolecules appeared first on Transfection Reagents |...| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems
Citations and References: ALTOGEN® Transfection Reagents Nature 2008 454(7203):523-7. Innate immunity induced by composition-dependent RIG-I …Saito et al [PDF] Nature Medicine 2016 22(10):1131–1139. A Long Non-Coding RNA Defines an Epigenetic Checkpoint … Wang et al [PDF] Cell Rep 2018 22(2):523. The Foxo1-Inducible Transcriptional Repressor Zfp125 … Gustavo et al [PDF] Molecular Therapy 2018. Liposome Lipid-Based […] The post Citations and References: Altogen® Transfection Kits a...| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems
LAS VEGAS, NEVADA – July 19, 2021 – Altogen Biosystems, leading manufacturer of in vivo transfection products, announced today that Altogen’s nanoparticle-based in vivoTransfection reagent (catalog #5031) was demonstrated to be effective for glioblastoma-targeted co-delivery of plasmid DNA (3.7 kb) and chemically modified siRNA. This transfection reagent was used in over 30 research publications, demonstrating highly […] The post In Vivo Nanoparticle Transfection Reagent is Effective...| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems
A stable cell line is a population of cells that have been genetically modified to express a specific gene or RNA molecule of interest on a stable basis. Stable cell lines are generated by introducing the gene or RNA molecule into the cells and selecting for cells that have stably incorporated the genetic material and […] The post Stable Cell Line appeared first on Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems.| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems
Transfection is considered as a major laboratory method to integrate protein, RNA and DNA molecules into tissues and cells. Delivery of plasmid DNA molecules containing gene inserts, messenger RNA and small interfering RNA molecules that regulate gene expression (i.e. microRNA, siRNA) into the primary cells and cancer cell lines have been extensively utilized by scientists. […] The post How do I increase transfection efficiency? appeared first on Transfection Reagents | Cell Lines, In Vivo ...| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems
July 27, 2021 – Altogen Biosystems® is a biotechnology manufacturing company that specializes in the development and testing of optimized cell transfection reagents and kits for both in vitro and in vivo delivery. In 2018 Altogen Biosystems launched the AltoFect transfection reagent formulated for difficult-to-transfect cells (like B-cells, T-cells), and primary cell cultures that are […] The post Crossing Blood-Brain Barrier: Delivery of Biomolecules into Glioblastoma Enabled by Altogen...| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems
The need to measure cell proliferation effects is vast, including testing the effects of growth factors, novel pharmacological agents, cytotoxicity assessment or investigating cell activation. Cell proliferation assays utilize a stain or substrate to make a correlation between the readout and the number of remaining cells post-treatment. However, the means of assessing cell number varies […] The post Quantitate in vitro anti-proliferation experiments using a metabolic assay (Alamarblue,...| Transfection Reagents | Cell Lines, In Vivo | Altogen Biosystems