What is an MYI File? MYI is also known as the MySQL MyISAM Index file. It is used to store indexes for the MyISAM table by MySQL. MySQL database index defines the structure of the table and contains the control mechanism to check the integrity of tables. MYI File Format MYI file has two parts, the header, and the key values. MYI Header The header contains information about options, file sizes, and keys.