PRAS40 and DEPTOR are mTOR binding proteins that affect cell metabolism. Under catabolic conditions such as sepsis and glucocorticoid excess, there is an increase in total DEPTOR protein and a reduction in phosphorylation of PRAS40, suggesting that these proteins may modulate the mTOR-mediated protein synthetic response under normal and diseased conditions. The hypothesis of the present study was that knock down (KD) of PRAS40 or DEPTOR in C2C12 myocytes will increase protein synthesis via ...
