This study explored how dilution protocols, enzymes, and solvents affected the performance of 2- and 3-phenyl substituted methylcarbamates with varying selectivity for Anopheles gambiae acetylcholinesterase (AgAChE). Protocol A was 100-fold suspension in buffer of a 0.1 M DMSO stock solution, followed by serial buffer dilutions to give declining DMSO in parallel with the inhibitor. Protocol B was identical to A except that the initial stock concentration was 0.01 M.
