The study analyzed the gut microbiota composition from over 3600 ambulatory donor samples undergoing routine medical evaluation from the USA, Canada, UK, Denmark, Australia, New Zealand, and other regions, spanning ages from 2 to over 70 years using the GA-map® multiplex polymerase chain reaction assay. The research aimed to identify differences in dysbiosis index (DI) score, diversity, and gastrointestinal (GI) microbiota across age categories and regions. Bacterial DNA was extracted, foll...