Coagulase Test: Principle, Applications, and Significance
Learn about the principle, applications, and significance of the coagulase test in this comprehensive guide.| microbialnotes.com
Learn about the principle, applications, and significance of the coagulase test in this comprehensive guide.| microbialnotes.com
Introduction Staphylococci are Gram-positive, spherical bacteria, approximately 1 µm in diameter, that typically appear in irregular clusters resembling grape bunches. The name originates from the Greek words staphyle (meaning “cluster of grapes”) and kokkos (meaning “berry”). There are at least 30 species of Staphylococcus that naturally inhabit the skin and mucous membranes, with some acting […]| microbialnotes.com
Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis, or SDS PAGE, is a method for sorting proteins according to their molecular weight| microbialnotes.com
Definition of Gel Electrophoresis SystemIt is a method used in laboratories that separates and analyses biomacromolecules including DNA, proteins, and RNA, according to their size and charge. Molecules transport across the gel, using an electric field, having smaller or differentially charged molecules moving faster and farther than bigger ones. It is a commonly used technique […] The post Important Parts Of Gel Electrophoresis System appeared first on Microbial notes.| Microbial notes
Introduction Using a laser energy-absorbing matrix, Matrix-Assisted Laser Desorption Ionization (MALDI), an ionization process used in mass spectrometry, produces ions from larger molecules with minimum fragmentation. It examines a wide range of organic compounds, such as dendrimers, polymers, and other macromolecules; also, it examines biomolecules, which are biopolymers that include proteins, peptides, polysaccharides, and DNA, […] The post A Comprehensive Description of MALDI TOF appeare...| Microbial notes
Introduction High-quality buffers are an essential component of electrophoresis. They allow a current to flow through the sample and resist pH changes in the surrounding solution. The isoelectric point of the sample determines the buffer used. The most often used buffers for DNA electrophoresis are TAE (Tris-acetate-EDTA) and TBE (Tris-borate-EDTA). SDS (sodium dodecyl sulfate) is a […]| Microbial notes
A Gram-negative bacterium that is a member of the Pseudomonadaceae family, Pseudomonas aeruginosa is very important in microbiology,| microbialnotes.com
Introduction Western blotting is a quick and very sensitive assay used in molecular biology. It is used for the characterization and detection of proteins. This method uses the inbuilt specificity of antigen-antibody interactions to identify specific antigens using polyclonal or monoclonal antibodies. Western blotting is also known as protein immunoblotting because an antibody is used selectively to […]| microbialnotes.com
Introduction S. typhi overview Salmonella typhi is a well-known human pathogen that is responsible for typhoid fever. It is a Gram-negative bacterium that is a member of the Enterobacteriaceae family. This particular bacterium distinguishes itself by targeting the human gastrointestinal tract with specificity.The result of a Salmonella typhi infection is typhoid fever, which is a […]| microbialnotes.com
The bacteria Salmonella Typhi, which is well-known for its effects on public health, is essential to epidemiology and scientific study| microbialnotes.com
Staphylococcus aureus Overview Staphylococcus aureus, commonly known as S. aureus, is a Gram-positive, facultative anaerobic bacterium belonging to the Staphylococcaceae family. Widely distributed in the environment and often found in colonizing the skin and mucous membranes of humans, S. aureus is a versatile microorganism with significant implications for both health and disease. As a crucial […] The post Isolation and Identification of Staphylococcus aureus appeared first on Microbial no...| Microbial notes
Definition The Acetamide Utilization Test is a biochemical test used in microbiology to determine the ability of microorganisms to utilize acetamide for bacterial growth and metabolism through the process of deamidation (an important metabolic pathway involving the conversion of acetamide). This biochemical test is specifically performed for the identification of aerobic microorganisms. Principal Media and […]| microbialnotes.com
Introduction Staphylococcus aureus, commonly known as S. aureus, is a significant bacterium that plays a crucial role in both the field of microbiology and its impact on human health. Named after its distinctive clustering shape resembling grapes, this bacterium is a part of the normal flora on the skin and mucous membranes of humans. While […]| microbialnotes.com
The presence or absence of either specific antibodies or antigens in a sample is measured and detected known as ELISA (Enzyme Linked Immunosorbent Assay).| microbialnotes.com
