Animal-derived components in cell culture, such as foetal bovine serum (FBS) and extracellular matrix proteins (ECM), pose ethical concerns and contribute to variability in experimental outcomes. This study explores the use of animal-free cell culture media and substrates to support the growth and differentiation of primary human bronchial epithelial cells (BECs), as well as their infection by respiratory syncytial virus (RSV). We evaluated the performance of jellyfish collagen 0 and recombin...