Biotin is a very commonly used label for oligonucleotides, thanks to its high affinity for streptavidin. This property makes it ideal for a wide range of experiments, and in this ‘Know Your Oligo Mod’ blog post we’ll explore some of the options available for biotin tags and their uses. Streptavidin is a bacterial protein that avidly binds biotin, an essential vitamin for many organisms. With a dissociation constant about 10-15 M, this is one of the strongest non-covalent interactions be...
